CORDIS Project
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This project aims to study the mechanisms of HIV Reverse Transcriptase at the single-molecule level using high-resolution optical tweezers. It seeks to understand the enzyme's polymerization process and its interactions with various factors, which could inform treatment strategies.
As the major target for therapy, Reverse Transcriptase (RT), and in particular the RT from the HIV virus, has been the subject of extensive research.
However, despite an ever-increasing amount of information from traditional “bulk” studies, the detailed mechanisms of polymerization are still not completely understood.
This is due, partially, to the stochastic nature of individual enzymes, which makes it impossible to follow more than one or two individual steps of polymerization using ensemble m…
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