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Chloro-Import investigates protein import pathways into chloroplasts in the green algae Chlamydomonas. The project aims to identify both canonical and non-canonical pathways using advanced genome-wide screening and biochemical methods, ultimately mapping the chloroplast protein-import system and enhancing our understan…
Protein import into a membrane-bound organelle in eukaryotes has been intensively studied, leading to the discovery of the central intracellular import machineries.
Yet, this field has undergone a fundamental paradigm shift following recent discoveries of non-canonical import pathways in the mitochondria and ER.
While the chloroplast is still thought to have but one import machinery (TIC/TOC), accumulating evidence indicate it, too, has non-canonical pathways.
Technological constraints have so f…
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