CORDIS Project
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This project investigates how proteins interact with nanoparticles in complex biological fluids, which can hinder diagnostic assays. By using DNA-assisted protein technology, it aims to understand protein coronas and their effects on nanoparticle behavior, ultimately improving point-of-care diagnostics.
Nanoparticles (from 1 to 1000 nM in diameter) are widely used in point-of-care diagnostics due to their large surface area and versatile biofunctionalization.
However, when used in matrices such as blood plasma and other complex biological fluids, nanoparticles are known to attract proteins that form coronas around the nanoparticles.
These coronas block binding sites, cause non-specific interactions, and cause aggregation among the nanoparticles, resulting in lower signals, higher background, an…
TECHNISCHE UNIVERSITEIT EINDHOVEN
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