CORDIS Project
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This project aims to enhance the observation of protein conformational dynamics by combining single-molecule Förster resonance energy transfer with nanopore-based electrophysiological techniques. This innovative approach seeks to extend observation times and integrate multiple measurements, specifically focusing on the…
The conformational dynamics of proteins are essential for their proper functioning, and impaired dynamics can lead to disease.
However, it remains difficult to experimentally determine the multitude of conformations adopted by proteins, their lifetimes and the transitions between them.
Single-molecule FRET (smFRET) is probably the most suitable technique available due to its ability to operate under ambient conditions while avoiding ensemble averaging.
Yet the technique is limited by the short o…
KATHOLIEKE UNIVERSITEIT LEUVEN
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