CORDIS Project
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This project innovates the crystal sponge method to analyze noncrystalline molecules using 3D electron diffraction. By developing new sponge materials, it aims to enhance the efficiency of structural analysis for complex lipids and other challenging analytes.
In the crystal sponge method (CSM), noncrystalline molecules are ordered bound in a porous crystal to allow collection of singlecrystal X-ray diffraction (SCXRD) data of both the host and the analyte.
This protocol has huge promise but is hindered by a lack ofsuitable sponge materials that can be isolated as large single crystals, and difficulties in inducing the ordered, high occupancy guestloading required for data collection.
Herein, we propose to apply the CSM to 3D electron diffraction (3DE…
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