CORDIS Project
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This project aims to enhance optical microscopy techniques to achieve resolutions better than 20 nanometers for studying protein arrangements in living cells. By applying advanced computational methods, it seeks to provide insights into molecular structures and dynamic processes.
Electron microscopy (EM) is an invaluable tool for investigating the nanometer-scale organization of molecular assemblies such as viruses, but is restricted to dead cells, does not readily label targeted proteins, and is prone to fixation artefacts.
Recently developed methods to break the diffraction limit in optical microscopy have the potential to resolve protein arrangements in living cells.
However, their resolution is currently restricted to ~20-30 nm, still an order of magnitude removed fr…
INSTITUT PASTEUR
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