CORDIS Project
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This project focuses on using aminoacyl transferases to enhance mass spectrometry techniques. It aims to develop methods for tagging proteins, which could improve the analysis of proteolysis products relevant to various diseases.
There are aminoacyl transferases, such as arginyl tRNA protein transferase 1 (Ate1) in Eukaryotes and leucyl/phenylalanyl tRNA protein transferase (L/F transferase) in Bacteria.
Both enzymes recognize specific N-terminal amino acids of a target poly-peptide and conjugate an amino acid from an aminoacylated tRNA onto the N-terminus of the targeted poly-peptide.Ate1 conjugates an arginyl moiety onto the N-terminus of a poly-peptide.
Electrospray ionization of peptides critically depends on argin…
EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
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