CORDIS Project
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This project focuses on improving electron cryomicroscopy techniques for studying protein complexes. It aims to enhance sample preparation by investigating how DNA origami structures influence grid preparation and to identify design improvements for these nano-tools.
Electron cryomicroscopy (cryo-EM) is rapidly emerging as a powerful technique for high-resolution structure determination of protein complexes.
Despite recent advances, there is still ample room for improvement of this method, in particular regarding sample preparation.
Outstanding challenges are reproducibility of freezing conditions to obtain an optimal ice thickness; to prevent unfolding or preferential orientation of proteins against the hydrophobic air-water interface; and a limited accurac…
UNITED KINGDOM RESEARCH AND INNOVATION
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