CORDIS Project
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This project explores in vitro selection techniques to enhance the development of functional biomolecules, particularly focusing on cellulases, which are crucial for biofuel production. It utilizes microfluidic devices to create water-in-oil emulsion droplets that facilitate the directed evolution of these enzymes.
In vitro selections offer the potential to select from large libraries, with fewer constraints than current cell-based selections.
The key practical consideration in directed evolution of functional biomolecules is the linkage of genotype and phenotype, which can be provided by water-in-oil emulsion droplets that act as femtolitre reactors.
Novel applications for catalytic selections of cellulases, key enzyme in teh production of bio-fuels, will be developed by in vitro compartmentalisation tec…
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