CORDIS Project
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This project aims to develop a novel experimental setup to measure the transfer of designed peptide sequences from water into lipid bilayers. By utilizing a modified peptide derived from bacteriorhodopsin, the research seeks to understand membrane protein folding mechanisms and improve computer simulation models in bio…
The transfer of peptide segments into the lipid bilayer to form stable transmembrane helices is the crucial first step in membrane protein folding and assembly.
However, the mechanisms that drive this process are not fully understood. A recent experimental assay has measured the insertion of designed peptide sequences into the endoplasmic reticulum membrane via the cellular translocon machinery.
This has provided the first quantitative estimate of the translocon-to-membrane transfer free energy…
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