CORDIS Project
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This project focuses on improving protein engineering through directed evolution techniques. It aims to utilize next-generation sequencing to analyze enzyme variants, enhancing the understanding of how mutations affect enzyme function and fitness.
Directed evolution is an effective method for protein engineering.
The host laboratory is designing innovative techniques for the directed evolution experiments of enzymes, with the possibility to test millions or billions of variants to be tested at each cycle.
In this project, I will introduce the use of full-gene next generation sequencing to supervise the exploration of variants at the sequence level and quantitatively characterize the fitness landscape, with a focus on non-additive effects…
ECOLE SUPERIEURE DE PHYSIQUE ET DECHIMIE INDUSTRIELLES DE LA VILLE DEPARIS
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