CORDIS Project
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LIMITLESS aims to develop a novel technology for whole-embryo genetic manipulation in mice, addressing limitations of traditional models. By leveraging advanced viral techniques and lineage tracing, the project seeks to enhance research capabilities in developmental biology and neuroscience.
Mouse models are a gold standard for life sciences research, spanning developmental biology, cancer studies, and pre-clinical testing, but they also have important limitations.
Mouse colony maintenance is expensive, and the large colonies needed are ethically problematic.
Approaches aiming for cell-type specific manipulations can be confounded by leaky promoters, off-target/unknown promoter expression, endogenous Cre sites, and Cre toxicity.
Finally, mouse strains generated in different inbred g…
KAROLINSKA INSTITUTET
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