CORDIS Project
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This project develops a cell-free protein translation system to incorporate unnatural amino acids into proteins, enhancing their properties for research applications. By utilizing evolved tRNA synthetases and suppressor-tRNA from Archaea, the project aims to overcome limitations in amino acid transport and toxicity, en…
The incorporation of unnatural amino acids (more than 50 to date) into proteins in vivo has resulted in the generation ofproteins with novel chemical, biological, and physical properties.
However, some unnatural amino acids possess properties,such as an inability to cross the cell membrane or a level of toxicity dangerous to the organism, that restrict their incorporationinto proteins in vivo.
In addition, even when an unnatural amino acid crosses the cell membrane, its transport efficiencywithi…
BEN-GURION UNIVERSITY OF THE NEGEV
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