CORDIS Project
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The project aims to develop thermothickening polymers that transition from low-viscosity liquids to hydrogels during PCR assays. This innovation will enhance the sensitivity and efficiency of DNA amplification, facilitating rapid testing in point-of-care devices.
The project aims at designing, synthesizing and optimizing thermothickening polymer compositions that would behave as liquid miscible with water, exhibit a low viscosity at room temperature and turn into a homogeneous hydrogel during PCR with MCT assay, with the limited diffusion of the DNA target and PCR products and high enough diffusion of PCR reagents to permit efficient DNA amplification.
For this purpose copolymers consisting of a water-soluble backbone with side chains exhibiting LCST wil…
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